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human tnf  (InvivoGen)


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    Structured Review

    InvivoGen human tnf
    Human Tnf, supplied by InvivoGen, used in various techniques. Bioz Stars score: 95/100, based on 104 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/recombinant+human+tnf+%CE%B1/Recombinant+human+TNF-%CE%B1/pm42152018-124-13-23
    Average 95 stars, based on 104 article reviews
    human tnf - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Recombinant:

    Article Title: Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasis
    Article Snippet: Sodium hydrosulfide (NaHS), morpholin- 4- ium 4- methoxphenyl(morpholino) phosphinodithioate dichloromethane complex (GYY4137), Phorbol- 12- myristate- 13- acetate (PMA), N- acetyl cysteine (NAC), Prostratin, DL- Propargylglycine (PAG), and L- Cysteine were purchased from Sigma- Aldrich. .. Recombinant human TNF-α was purchased from InvivoGen. ..

    Article Title: Effect of Total Flavones of Astragalus on Apoptosis and Inflammation of Alveolar Epithelial Cells Induced by Tumor Necrosis Factor Alpha through microRNA-221
    Article Snippet: .. Materials: Human alveolar epithelial cells were purchased from ScienCell, United States of America (USA); Dulbecco's Modified Eagle Medium (DMEM) medium was purchased from HycloneTM, USA; recombinant human TNF-α was purchased from Invivogen, USA; total flavones of Astragalus were purchased from Shanghai Ziqibio Co., Ltd.; miRNA reverse transcription kit was purchased from GeneCopoeia, USA; miRNA fluorescence qualitative Polymerase Chine Reaction (qPCR) kit was purchased from Shanghai Genepharma Co., Ltd.; (3-(4,5-Dimethylthiazol-2-yl)-2,5- Diphenyltetrazolium Bromide (MTT) kit, Annexin V-Fluorescein Isothiocyante (FITC)/Propidium Iodide (PI) apoptosis detection kit were purchased from Wuhan Amyjet Scientific Inc.; protein extraction kits were purchased from Wuhan Chundu Biotechnology Co., Ltd.; Interleukin-1 (IL)-1 beta (β), IL-6 and IL-10 assay kits were purchased from Beijing Baio Leibo Technology Co., Ltd. ..

    Article Title: A scalable long-term in vitro model of human colonic epithelium with continuous barrier function
    Article Snippet: .. The addition of cytokines for barrier challenge occurred on day 8 and included recombinant human TNF-α (5 ng/mL, InvivoGen rcyc-htnfa) and/or recombinant human IFN-γ (5 ng/mL, InvivoGen rcyec-hifng) diluted in the basolateral medium where the Clostridium difficile Toxin B (TcdB; 10 ng/mL, Sigma-Aldrich SML1153) and EDTA (2mM) were diluted in the apical medium. ..

    Article Title: Small molecule SJ572946 activates BAK to initiate apoptosis
    Article Snippet: SYTOX Green , Thermo Fisher Scientific , Cat# S7020 CAS# 163795-75-3. .. Recombinant human TNF-α , Invivogen , Cat# Rhtnf-a. .. Recombinant human sTRAIL/Apo2L , Peprotech , Cat# 10770-798.

    Article Title: Borrelia burgdorferi modulates the physical forces and immunity signaling in endothelial cells
    Article Snippet: Propidium iodide , Sigma , Cat# P4170. .. Recombinant human TNF-α , InvivoGen , Cat# rcyc-htnfa. .. Muramyldipeptide - MDP | NOD2 ligand , InvivoGen , Cat# tlrl-mdp.

    Article Title: Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasis
    Article Snippet: Sodium hydrosulfide (NaHS), morpholin-4-ium 4-methoxphenyl(morpholino) phosphinodithioate dichloromethane complex (GYY4137), Phorbol-12-myristate-13-acetate (PMA), N-acetyl cysteine (NAC), prostratin and L-Cysteine were purchased from Sigma-Aldrich. .. Recombinant human TNF- α was purchased from InvivoGen. ..

    Article Title: Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasis
    Article Snippet: Sodium hydrosulfide (NaHS), morpholin-4-ium 4-methoxphenyl(morpholino) phosphinodithioate dichloromethane complex (GYY4137), Phorbol-12-myristate-13-acetate (PMA), N-acetyl cysteine (NAC), Prostratin, DL-Propargylglycine (PAG), and L-Cysteine were purchased from Sigma-Aldrich. .. Recombinant human TNF-α was purchased from InvivoGen. ..

    Article Title: Programming DNA Circuits for Controlled Immunostimulation through CpG Oligodeoxynucleotide Delivery.
    Article Snippet: Herein, we present a DNA circuit programmed for the delivery of CpG oligodeoxynucleotides (CpG ODNs) with the pharmacological immunostimulation function.. The circuit employs a complementary DNA (cDNA) strand to deactivate the biological function of CpG ODNs via hybridization, while T7 exonuclease mediates the activation by hydrolyzing the cDNA and releasing the CpG ODN as an active moiety.. We investigated the influence of several factors on the kinetic profile and temporal behavior of the circuit.

    Modification:

    Article Title: Effect of Total Flavones of Astragalus on Apoptosis and Inflammation of Alveolar Epithelial Cells Induced by Tumor Necrosis Factor Alpha through microRNA-221
    Article Snippet: .. Materials: Human alveolar epithelial cells were purchased from ScienCell, United States of America (USA); Dulbecco's Modified Eagle Medium (DMEM) medium was purchased from HycloneTM, USA; recombinant human TNF-α was purchased from Invivogen, USA; total flavones of Astragalus were purchased from Shanghai Ziqibio Co., Ltd.; miRNA reverse transcription kit was purchased from GeneCopoeia, USA; miRNA fluorescence qualitative Polymerase Chine Reaction (qPCR) kit was purchased from Shanghai Genepharma Co., Ltd.; (3-(4,5-Dimethylthiazol-2-yl)-2,5- Diphenyltetrazolium Bromide (MTT) kit, Annexin V-Fluorescein Isothiocyante (FITC)/Propidium Iodide (PI) apoptosis detection kit were purchased from Wuhan Amyjet Scientific Inc.; protein extraction kits were purchased from Wuhan Chundu Biotechnology Co., Ltd.; Interleukin-1 (IL)-1 beta (β), IL-6 and IL-10 assay kits were purchased from Beijing Baio Leibo Technology Co., Ltd. ..

    Reverse Transcription:

    Article Title: Effect of Total Flavones of Astragalus on Apoptosis and Inflammation of Alveolar Epithelial Cells Induced by Tumor Necrosis Factor Alpha through microRNA-221
    Article Snippet: .. Materials: Human alveolar epithelial cells were purchased from ScienCell, United States of America (USA); Dulbecco's Modified Eagle Medium (DMEM) medium was purchased from HycloneTM, USA; recombinant human TNF-α was purchased from Invivogen, USA; total flavones of Astragalus were purchased from Shanghai Ziqibio Co., Ltd.; miRNA reverse transcription kit was purchased from GeneCopoeia, USA; miRNA fluorescence qualitative Polymerase Chine Reaction (qPCR) kit was purchased from Shanghai Genepharma Co., Ltd.; (3-(4,5-Dimethylthiazol-2-yl)-2,5- Diphenyltetrazolium Bromide (MTT) kit, Annexin V-Fluorescein Isothiocyante (FITC)/Propidium Iodide (PI) apoptosis detection kit were purchased from Wuhan Amyjet Scientific Inc.; protein extraction kits were purchased from Wuhan Chundu Biotechnology Co., Ltd.; Interleukin-1 (IL)-1 beta (β), IL-6 and IL-10 assay kits were purchased from Beijing Baio Leibo Technology Co., Ltd. ..

    Fluorescence:

    Article Title: Effect of Total Flavones of Astragalus on Apoptosis and Inflammation of Alveolar Epithelial Cells Induced by Tumor Necrosis Factor Alpha through microRNA-221
    Article Snippet: .. Materials: Human alveolar epithelial cells were purchased from ScienCell, United States of America (USA); Dulbecco's Modified Eagle Medium (DMEM) medium was purchased from HycloneTM, USA; recombinant human TNF-α was purchased from Invivogen, USA; total flavones of Astragalus were purchased from Shanghai Ziqibio Co., Ltd.; miRNA reverse transcription kit was purchased from GeneCopoeia, USA; miRNA fluorescence qualitative Polymerase Chine Reaction (qPCR) kit was purchased from Shanghai Genepharma Co., Ltd.; (3-(4,5-Dimethylthiazol-2-yl)-2,5- Diphenyltetrazolium Bromide (MTT) kit, Annexin V-Fluorescein Isothiocyante (FITC)/Propidium Iodide (PI) apoptosis detection kit were purchased from Wuhan Amyjet Scientific Inc.; protein extraction kits were purchased from Wuhan Chundu Biotechnology Co., Ltd.; Interleukin-1 (IL)-1 beta (β), IL-6 and IL-10 assay kits were purchased from Beijing Baio Leibo Technology Co., Ltd. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Effect of Total Flavones of Astragalus on Apoptosis and Inflammation of Alveolar Epithelial Cells Induced by Tumor Necrosis Factor Alpha through microRNA-221
    Article Snippet: .. Materials: Human alveolar epithelial cells were purchased from ScienCell, United States of America (USA); Dulbecco's Modified Eagle Medium (DMEM) medium was purchased from HycloneTM, USA; recombinant human TNF-α was purchased from Invivogen, USA; total flavones of Astragalus were purchased from Shanghai Ziqibio Co., Ltd.; miRNA reverse transcription kit was purchased from GeneCopoeia, USA; miRNA fluorescence qualitative Polymerase Chine Reaction (qPCR) kit was purchased from Shanghai Genepharma Co., Ltd.; (3-(4,5-Dimethylthiazol-2-yl)-2,5- Diphenyltetrazolium Bromide (MTT) kit, Annexin V-Fluorescein Isothiocyante (FITC)/Propidium Iodide (PI) apoptosis detection kit were purchased from Wuhan Amyjet Scientific Inc.; protein extraction kits were purchased from Wuhan Chundu Biotechnology Co., Ltd.; Interleukin-1 (IL)-1 beta (β), IL-6 and IL-10 assay kits were purchased from Beijing Baio Leibo Technology Co., Ltd. ..

    MTT Assay:

    Article Title: Effect of Total Flavones of Astragalus on Apoptosis and Inflammation of Alveolar Epithelial Cells Induced by Tumor Necrosis Factor Alpha through microRNA-221
    Article Snippet: .. Materials: Human alveolar epithelial cells were purchased from ScienCell, United States of America (USA); Dulbecco's Modified Eagle Medium (DMEM) medium was purchased from HycloneTM, USA; recombinant human TNF-α was purchased from Invivogen, USA; total flavones of Astragalus were purchased from Shanghai Ziqibio Co., Ltd.; miRNA reverse transcription kit was purchased from GeneCopoeia, USA; miRNA fluorescence qualitative Polymerase Chine Reaction (qPCR) kit was purchased from Shanghai Genepharma Co., Ltd.; (3-(4,5-Dimethylthiazol-2-yl)-2,5- Diphenyltetrazolium Bromide (MTT) kit, Annexin V-Fluorescein Isothiocyante (FITC)/Propidium Iodide (PI) apoptosis detection kit were purchased from Wuhan Amyjet Scientific Inc.; protein extraction kits were purchased from Wuhan Chundu Biotechnology Co., Ltd.; Interleukin-1 (IL)-1 beta (β), IL-6 and IL-10 assay kits were purchased from Beijing Baio Leibo Technology Co., Ltd. ..

    Protein Extraction:

    Article Title: Effect of Total Flavones of Astragalus on Apoptosis and Inflammation of Alveolar Epithelial Cells Induced by Tumor Necrosis Factor Alpha through microRNA-221
    Article Snippet: .. Materials: Human alveolar epithelial cells were purchased from ScienCell, United States of America (USA); Dulbecco's Modified Eagle Medium (DMEM) medium was purchased from HycloneTM, USA; recombinant human TNF-α was purchased from Invivogen, USA; total flavones of Astragalus were purchased from Shanghai Ziqibio Co., Ltd.; miRNA reverse transcription kit was purchased from GeneCopoeia, USA; miRNA fluorescence qualitative Polymerase Chine Reaction (qPCR) kit was purchased from Shanghai Genepharma Co., Ltd.; (3-(4,5-Dimethylthiazol-2-yl)-2,5- Diphenyltetrazolium Bromide (MTT) kit, Annexin V-Fluorescein Isothiocyante (FITC)/Propidium Iodide (PI) apoptosis detection kit were purchased from Wuhan Amyjet Scientific Inc.; protein extraction kits were purchased from Wuhan Chundu Biotechnology Co., Ltd.; Interleukin-1 (IL)-1 beta (β), IL-6 and IL-10 assay kits were purchased from Beijing Baio Leibo Technology Co., Ltd. ..



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    Image Search Results


    Detection of inflammatory cytokines in skin wash of healthy volunteers and patients with AD and psoriasis. Cytokine concentrations were measured in skin wash fluid using Luminex assay. Inflammatory cytokines ( a ) IL-1β, ( b ) TNFα, ( c ) IL-18, and ( d ) IL-8. AD-related immune mediators ( e ) CCL13, ( f ) CCL18, ( g ) sIL-2R, and ( h ) IL-4. Psoriasis-associated immune mediators ( i ) IL-17A, ( j ) IL-23, ( k ) sTNFR1, and ( l ) IFNγ. Concentrations are depicted as mean ± SD, with individual data points shown. PS denotes psoriasis, NL denotes nonlesional, and L denotes lesional. ∗ P < .05, ∗∗ P < .01, and ∗∗∗ P < .001. AD, atopic dermatitis; HC, healthy control; sIL-2R, soluble IL-2R; sTNFR1, soluble TNFR1.

    Journal: JID Innovations

    Article Title: Development of a noninvasive device and method for measuring immune parameters in skin disease

    doi: 10.1016/j.xjidi.2026.100456

    Figure Lengend Snippet: Detection of inflammatory cytokines in skin wash of healthy volunteers and patients with AD and psoriasis. Cytokine concentrations were measured in skin wash fluid using Luminex assay. Inflammatory cytokines ( a ) IL-1β, ( b ) TNFα, ( c ) IL-18, and ( d ) IL-8. AD-related immune mediators ( e ) CCL13, ( f ) CCL18, ( g ) sIL-2R, and ( h ) IL-4. Psoriasis-associated immune mediators ( i ) IL-17A, ( j ) IL-23, ( k ) sTNFR1, and ( l ) IFNγ. Concentrations are depicted as mean ± SD, with individual data points shown. PS denotes psoriasis, NL denotes nonlesional, and L denotes lesional. ∗ P < .05, ∗∗ P < .01, and ∗∗∗ P < .001. AD, atopic dermatitis; HC, healthy control; sIL-2R, soluble IL-2R; sTNFR1, soluble TNFR1.

    Article Snippet: E coli –derived recombinant human TNFα, IL-1β, IL-6, IL-8, IL-10, and IL-18 (endotoxin levels <0.10 EU/μg by the limulus amebocyte lysate method) were purchased from R&D Systems and reconstituted in PBS.

    Techniques: Luminex, Control

    Effects on IL-1β (A) , IL-6 (B) , and IL-8 (C) levels in HFLS-RA cells induced by TNF-α (5 ng/mL). n = 3; vs. 0 h, ** p < 0.01; **** p < 0.0001.

    Journal: Frontiers in Medicine

    Article Title: Identification of diagnostic genes in rheumatoid arthritis using integrated bioinformatics, machine learning, and experimental validation

    doi: 10.3389/fmed.2026.1803764

    Figure Lengend Snippet: Effects on IL-1β (A) , IL-6 (B) , and IL-8 (C) levels in HFLS-RA cells induced by TNF-α (5 ng/mL). n = 3; vs. 0 h, ** p < 0.01; **** p < 0.0001.

    Article Snippet: Upon reaching approximately 70% confluence, cells were stimulated with 5 ng/mL recombinant human TNF-α (Servicebio, China) to establish an in vitro rheumatoid arthritis model.

    Techniques:

    Quantitative analysis of MAGED1 (A) , EPYC (B) , and LAP3 (C) mRNA expression in HFLS-RA induced by TNF-α (5 ng/mL). n = 3; vs. 0 h, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Journal: Frontiers in Medicine

    Article Title: Identification of diagnostic genes in rheumatoid arthritis using integrated bioinformatics, machine learning, and experimental validation

    doi: 10.3389/fmed.2026.1803764

    Figure Lengend Snippet: Quantitative analysis of MAGED1 (A) , EPYC (B) , and LAP3 (C) mRNA expression in HFLS-RA induced by TNF-α (5 ng/mL). n = 3; vs. 0 h, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.

    Article Snippet: Upon reaching approximately 70% confluence, cells were stimulated with 5 ng/mL recombinant human TNF-α (Servicebio, China) to establish an in vitro rheumatoid arthritis model.

    Techniques: Expressing